Screening of Bioactive Secondary Metabolites of Streptomyces spp. Isolated from the Sediments

Abstract

The purpose of this study was to assess the activity of Streptomyces spp., isolate and identify them from samples of silt soil and Tigris river water in Mosul, Iraq. Variations in the isolates' microscopic and biochemical properties were used to identify them., such as soluble pigment, aerobic mycelium, and substrate mycelium. Using the perpendicular streak method, eight of the twelve actinomycete isolates that were isolated from the water and sediments of the Tigris River exhibited antibacterial activity against specific pathogenic bacteria. Staphyloccocus aureus (MTCC 3260), Vibrio parahaemolyticus, Klibsiella pneumoniae, Salmonella typhi, Proteus mirabilis, E. coli (MTCC 443), Pseudomonas aeruginosa (MTCC 424), and Aeromonas hydrophila bacteria were all susceptible to the broad-spectrum activity of isolate Sediment Lake Iraq E2. According to the findings of phylogenetic analysis, isolate E2 shared the closest kinship with Streptomyces sp. (EU257231). Using high-performance liquid chromatography (HPLC), the Streptomyces isolate E2's secondary metabolite was further examined. There is evidence that these secondary metabolites are harmless and may have antibacterial properties against human pathogens.