Acute 60Co Γ-Irradiation Effect on Lipid Peroxidation and Immune System in Experimental Animals

Abstract

The objective of the study was to explore the biochemical and immunological indicators in experimentally irradiated animals. Goup I - intact, group II - exposed to γ-radiation with a dose 6 Gy. The parameters characterizing the body's immune system were studied, lipid peroxidation outputs and antioxidant protective enzymes activity in blood lymphocytes were identified. The results of the study showed that exposure to a sublethal dose of radiation entails a reduction in cellular immunity, especially T-lymphocytes and their subpopulation, as well as to a decrease in the functional activity of the body's non-specific defense. Leukopenia and lymphopenia were observed in irradiated animals. The same pattern was revealed for T-lymphocytes: the number decreased by 20.25%, leading to fell off in CD4+ cells. It was also found that T-suppressors number declined by 14.28%. Ionizing radiation effect led to an increase in the level of DC and MDA, inhibition of the activity of glutathione reductase, glutathione peroxidase and catalase enzymes, as a result of which the oxidative stress formation in the body was observed. Suppression of cellular and humoral immunity, non-specific protection of the body and imbalance of LPO-AOP create preconditions for the emergence of immune pathological state of radiation origin, indicating the necessity to develop promising for adaptation correcting methods.